RNA Sequencing

RNA sequencing is becoming an increasingly powerful tool for deepening our understanding of biological processes. It provides unprecedented sensitivity and accuracy for analyzing gene expression, and has opened new doors for researchers conducting a wide range of studies. We provide flexible RNA sequencing options – from small RNA and miRNA sequencing, to sequencing of the coding transcriptome and the whole transcriptome. Customized options are available upon request.

Options

Whole Transcriptome mRNA Sequencing

Capture is by Poly(dT) oligomers which relies on good quality RNA, and is available for non-degraded samples only.

rRNA Depleted Total RNA Sequencing

Selective removal of ribosomal RNA (rRNA) allows for a more efficient analysis in degraded/low quality samples. This option is recommended as last resort for analysis of FFPE samples.

Turnaround Time
  • Minimum Order Quantity: 8 samples
  • Turnaround Time: 3-4 weeks
  • Minimum Order Quantity: 8 samples
  • Turnaround Time: 3-4 weeks
Services Included
  • Extraction
  • Library Prep
  • Sequencing
  • Extraction
  • Library Prep
  • Sequencing
  • rRNA removal
Deliverables
  • FastQ
  • BAM
  • QC Report
  • FastQ
  • BAM
  • QC Report
Species
  • Human
  • Mouse
  • Human
  • Mouse
Technical Specifications

Please contact us for custom requests.

  • 30M read pairs
  • Paired-end 2x150 bp
  • 30M read pairs
  • Paired-end 2x150 bp
Sample Requirements
  • Whole Blood, Extracted Total RNA, Cell lines
  • Amount: Minimum 400ng Total RNA
  • Specimens: contact for details
  • RNA Purity: OD260/OD280 > 1.8
  • RIN > 8.0
  • Whole Blood, Extracted Total RNA, Cell lines
  • Amount: Minimum 400ng Total RNA
  • Specimens: contact for details
  • RNA Purity: OD260/OD280 > 1.8
  • RIN > 2.0
Options

Our most requested RNA sequencing service. The coding transcriptome represents all coding regions of mRNA molecules within a cell at any given moment. Coding regions are selectively targeted, allowing the capture of specific transcripts. This method is good for both high quality and degraded RNA.

Target Capture RNA Sequencing - 30M reads

Turnaround Time
  • Minimum Order Quantity: 8 samples
  • Turnaround Time: 3-4 weeks
Services Included
  • Library Prep
  • Sequencing
  • Extraction
Deliverables
  • FastQ
  • BAM
  • QC Report
Species
  • Human
  • Mouse
Technical Specifications

Please contact us for custom requests.

  • 30M read pairs
  • Paired-end 2x150 bp
  • Whole Exome probe capture
  • Specific panel probes
Sample Requirements
  • Whole Blood, DNA, Buccal Swab, Saliva, Cell lines
  • FFPE: Contact for details and quality requirements
  • Amount: Minimum 500ng DNA
  • Specimens: contact for details
  • DNA Purity: OD260/OD280 > 1.8
Options

Small RNA Sequencing

Small interfering RNA (Si-RNA) are small double-stranded RNA molecules, typically 21-25 base-pairs in length. Like miRNA, Si-RNA play a central role in RNA interference.

miRNA Sequencing

Micro-RNA (miRNA) are a class of small non-coding, single-stranded RNAs around 21-25 nucleotides in length. These small molecules bind to mRNA through complementary base-pairing, and play an active role in post-transcriptional regulation of gene expression.

Turnaround Time
  • Minimum Order Quantity: 20 samples
  • Turnaround Time: 4-5 weeks
  • Minimum Order Quantity: 20 samples
  • Turnaround Time: 4-5 weeks
Services Included
  • Library Prep
  • Sequencing
  • Library Prep
  • Sequencing
Deliverables
  • FastQ
  • FastQ QC
  • FastQ
  • FastQ QC
Technical Specifications
  • 15M reads
  • Single read 1x40 bp
  • 15M reads
  • Single read 1x40 bp
Sample Requirements
  • Whole Blood, Extracted Total RNA
  • Amount: Minimum 200ng Total RNA
  • Specimens: contact for details
  • RNA Purity: OD260/OD280 > 1.8
  • RIN > 8.0
  • Whole Blood, Extracted Total RNA
  • Amount: Minimum 200ng Total RNA
  • Specimens: contact for details
  • RNA Purity: OD260/OD280 > 1.8
  • RIN > 8.0

Sample Output

Make a request if you would like to view our sample QC and Coverage reports.

Delivery Format

Your data privacy and security is our priority. That's why your data will be delivered to you personally through our Fulgent Cloud. We utitlize a FTP that uses TLS/SSL certificates.